Plasmid

Part:BBa_K4876010:Design

Designed by: Jin Luming   Group: iGEM23_Zhejiang-United   (2023-09-28)


p15A-cas9-op-λ-Red


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6863
    Illegal NheI site found at 9344
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3244
    Illegal BglII site found at 3703
    Illegal BglII site found at 5632
    Illegal BamHI site found at 5434
    Illegal BamHI site found at 11620
    Illegal BamHI site found at 12480
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5705
    Illegal NgoMIV site found at 12506
    Illegal AgeI site found at 1397
    Illegal AgeI site found at 6306
    Illegal AgeI site found at 6949
    Illegal AgeI site found at 7273
    Illegal AgeI site found at 12829
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2225
    Illegal SapI.rc site found at 5125


Design Notes

NO


Source

NO

References